RADOV
RaDiation harvesting of bioactive peptides from egg prOteins and their integration in adVanced functional products
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WP2: Development of laboratory scale production of irradiated egg products and peptides
Objectives:
1. To develop protocols for in-vitro bioactivity evaluation of irradiated egg proteins and egg protein-derived peptides.
2. To identify bioactive products from irradiation of egg protein mixtures and isolates.
3. To develop isolation procedures and methods for structural identification of egg protein-derived peptides.
4. To determine optimal irradiation conditions for the production of egg-protein fractions and peptides with antibacterial and antioxidant properties to be included in products developed in WP3.
5. To identify relationships between chemical structure of products from egg protein irradiation and their bioactivity innovation management.
Tasks:
2.1 Laboratory scale irradiation of eggshell membrane (ESM), egg white (EW) and isolated egg proteins.
2.2 Development of in vitro bioactive properties evaluation protocols and bioactivity evaluation of
irradiated egg proteins and irradiation-produced peptides.
2.3 Development of isolation procedures, structural identification and quantification of target fractions and/or peptides in irradiated materials.
2.4 Development of structure-process and structure-activity database, processing conditions
optimisation and irradiated materials selection.